Isopycnic density-gradient centrifugation: A separation parameter which improves flow cytometric measurements on heterogeneous tumors
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Separation of Hela Cells by Colloidal Silica Density Gradient Centrifugation
Using a colloidal silica density gradient, HeLa cells in mitosis were found to have a density of 1.040-1.046 g/cc, lighter than the remaining interphase cells. The mitotic cells could be harvested and cultured after centrifugation, showing growth synchrony by measurement of a peak in mitotic index 21 hr after establishing the culture. By using Colcemid or vinblastine sulfate, HeLa cells were ar...
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Neurath, A. Robert (The Wistar Institute, Philadelphia, Pa.), Tadeusz J. Wiktor, and Hilary Koprowski. Density gradient centrifugation studies on rabies virus. J. Bacteriol. 92:102-106. 1966.-Cesium chloride density gradient centrifugation of rabies virus revealed a heterogeneous population of infectious virus particles, the majority of which showed a density of 1.20 g/ml. From results obtained...
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NEURATH, A. ROBERT (The Wistar Institute, Philadelphia, Pa.), TADEUSZ J. WIKTOR, AND HILARY KoPROWSKI. Density gradient centrifugation studies on rabies virus. J. Bacteriol. 92:102-106. 1966.-Cesium chloride density gradient centrifugation of rabies virus revealed a heterogeneous population of infectious virus particles, the majority of which showed a density of 1.20 g/ml. From results obtained...
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background: it has been claimed that by using different washing methods, the sperms can be separated according to size, motility, density, chromosomal content and surface markings and charge. these methods also reduce sperm chromatin deficiencies and screen the sperms before applying in assisted reproduction techniques. objective: this study compared simple density gradient methods and a combin...
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Fritsch, A. (1973) Anal. Biochem. 35,57-71 Griffith, 0. M. (1978) Anal. Biochem. 9 0 , 4 3 5 4 4 3 Hames, B. D. & Rickwood, D. (1978) in Centr&gation-A Practical Approach (Rickwood, D., ed.), pp. 69-80, Information Retrieval. London Ridge, D. (1978) in Centrifugal Separations in Molecular and Cell Biology (Birnie, G. D. & Rickwood, D., eds.), pp. 33-61. Butterworths, London Young, B. D. (1978) ...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1983
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990040207